公猪7号染色体繁殖性状QTL的精细定位和位置候选基因MEA1的鉴别研究

doi:10.11843/j.issn.0366-6964.2014.03.004

Abstract

Abstract:

This aim of this study was to fine map the QTL affecting boar epididymal weight and serum testosterone concentration on SSC7 and to identify the positional candidate genes affecting boar reproduction traits.The materials used in this study were 205 F₂ boars from a White Duroc× Erhualian resource population.For fine mapping the QTL affecting epididymal weight，14 SNPs in the region preliminarily mapped were increased as markers to scan the boar population.Male-enhanced antigen 1 (MEA1) was chosen as a positional candidate gene in the fine mapping region.Association and F-drop analyses were implemented to reveal the relationship between MEA1 polymorphisms and boar reproduction traits.RT-PCR and differential expression assays were used to detect the MEA1 expression.The results showed that：①Fine mapping analyses revealed that the QTL region affecting epididymal weight and serum testosterone concentration was significantly narrowed.② Association analyses revealed that five SNPs of MEA1 were extremely significantly or significantly associated with boar epididymal weight，serum testosterone concentration and semen volume (P<0.01 or P<0.05).G-C-C-G-C haplotype was extremely significantly associated with boar left and right epididymal weight (P<0.01)，and A-C-C-G-G haplotype was significantly associated with boar left and right epididymal weight (P<0.05).F-drop test showed that g.-1168A>G and g.1115A>G could be quantitative trait nucleotides (QTN) or be complete linkage disequilibrium with QTN affecting testosterone concentration.Differential expression analysis showed that epididymal weight had negative relationship with MEA1 expression level in this tissue (r²=-0.300,P=0.1).qRT-PCR assay detected the expression of MEA1 gene in all 18 selected tissues of a mature boar.QTL regions affecting epididymal weight and serum testosterone concentration were narrowed through fine mapping.Significant association was found between MEA1 gene and boar epididymal weight，serum testosterone concentration and semen volume.g.-1168 A>G and g.1115 A>G of MEA1 could be QTN or be complete linkage disequilibrium with QTN for boar serum testosterone concentration，and these two SNPs can be used as molecular markers for selecting boars with high libido.

CLC Number:

S828
S813.3

ZHAO Ke-wei，ZHAO Xue-yan，ZHANG Zhi-yan，HONG Yuan，FAN Yin，REN Jun，XING Yu-yun. Fine Mapping of QTL and Identification of MEA1 as a Positional Candidate Gene Affecting Boar Reproduction Traits on SSC7[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 363-374.

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Fine Mapping of QTL and Identification of MEA1 as a Positional Candidate Gene Affecting Boar Reproduction Traits on SSC7

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